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plv exp puroef1a egfp 3xflag  (Addgene inc)


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    Structured Review

    Addgene inc plv exp puroef1a egfp 3xflag
    Plv Exp Puroef1a Egfp 3xflag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 13825 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plv exp puroef1a egfp 3xflag/product/Addgene inc
    Average 98 stars, based on 13825 article reviews
    plv exp puroef1a egfp 3xflag - by Bioz Stars, 2026-03
    98/100 stars

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    Image Search Results


    RMND5A inhibits the proliferation, migration and tube formation of HUVECs. ( a , b ) Overexpression of Flag-tag RMND5A in HUVECs via lentiviral infection. Real-time PCR ( a ) and WB ( b ) data proves the elevated expression of RMND5A in HUVECs. ( c ) MTT assay shows ECGs dependent growth of HUVECs. By comparison with vector control group, overexpression of RMND5A significantly inhibits the proliferation of HUVECs under the condition of ECGs. ( d ) Would healing assay shows that overexpression of RMND5A significantly inhibited the migration ability of HUVECs. ( e ) Overexpression of RMND5A reduces the tube formation ability of HUVECs evidenced by decreased capillary-like structures. Data is expressed as mean ± SD. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001

    Journal: BMC Oral Health

    Article Title: Oral cancer cell to endothelial cell communication via exosomal miR-21/RMND5A pathway

    doi: 10.1186/s12903-024-03852-3

    Figure Lengend Snippet: RMND5A inhibits the proliferation, migration and tube formation of HUVECs. ( a , b ) Overexpression of Flag-tag RMND5A in HUVECs via lentiviral infection. Real-time PCR ( a ) and WB ( b ) data proves the elevated expression of RMND5A in HUVECs. ( c ) MTT assay shows ECGs dependent growth of HUVECs. By comparison with vector control group, overexpression of RMND5A significantly inhibits the proliferation of HUVECs under the condition of ECGs. ( d ) Would healing assay shows that overexpression of RMND5A significantly inhibited the migration ability of HUVECs. ( e ) Overexpression of RMND5A reduces the tube formation ability of HUVECs evidenced by decreased capillary-like structures. Data is expressed as mean ± SD. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001

    Article Snippet: Human RMND5A cDNA (NM_022780.4) was amplified by PCR and subcloned into a lentiviral pHS-AVC vector (pLV-hef1a-mNeongreen-P2A-Puro-WPRE-CMV-3xFlag) containing 3xFlag (Beijing Syngentech Co., Ltd.) (Beijing, China).

    Techniques: Migration, Over Expression, FLAG-tag, Infection, Real-time Polymerase Chain Reaction, Expressing, MTT Assay, Comparison, Plasmid Preparation