Journal: BMC Oral Health
Article Title: Oral cancer cell to endothelial cell communication via exosomal miR-21/RMND5A pathway
doi: 10.1186/s12903-024-03852-3
Figure Lengend Snippet: RMND5A inhibits the proliferation, migration and tube formation of HUVECs. ( a , b ) Overexpression of Flag-tag RMND5A in HUVECs via lentiviral infection. Real-time PCR ( a ) and WB ( b ) data proves the elevated expression of RMND5A in HUVECs. ( c ) MTT assay shows ECGs dependent growth of HUVECs. By comparison with vector control group, overexpression of RMND5A significantly inhibits the proliferation of HUVECs under the condition of ECGs. ( d ) Would healing assay shows that overexpression of RMND5A significantly inhibited the migration ability of HUVECs. ( e ) Overexpression of RMND5A reduces the tube formation ability of HUVECs evidenced by decreased capillary-like structures. Data is expressed as mean ± SD. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001
Article Snippet: Human RMND5A cDNA (NM_022780.4) was amplified by PCR and subcloned into a lentiviral pHS-AVC vector (pLV-hef1a-mNeongreen-P2A-Puro-WPRE-CMV-3xFlag) containing 3xFlag (Beijing Syngentech Co., Ltd.) (Beijing, China).
Techniques: Migration, Over Expression, FLAG-tag, Infection, Real-time Polymerase Chain Reaction, Expressing, MTT Assay, Comparison, Plasmid Preparation